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T7-p73DD L371P-pcDNA3

Plasmid

#23049

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p73 (TP73 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 327-…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-6xGLY-T7-HIS3MX6

Plasmid

#20759

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-T7-hAIM2

Plasmid

#51541

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

GFP and T7

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET31b-T7-Spinach2-Dp

Plasmid

#79159

Purpose

Expresses the Dp biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Dp

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-A

Plasmid

#79160

Purpose

Expresses the Pl-A biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-A

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 BimL (T56D)

Plasmid

#24532

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56D) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S58A)

Plasmid

#24249

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S58A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
T7 Lig-C-HiBiT

Plasmid

#215441

Purpose

Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag

Use

Tags

10xHis-tag with thrombin site and HiBiT Tag

Expression

Bacterial

Mutation

Codon Optimized for bacterial expression, T7 gene…

Promoter

T7 Promotor

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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T7 Lig-N-HiBiT

Plasmid

#215479

Purpose

Bacterially expressed T7 Lig-N-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Escherichia phage T7 DNA Ligase, N-terminal HiBiT, N-terminal 10XHis.Thrombin tag

Use

Tags

10xHis-tag with thrombin site and HiBiT tag

Expression

Bacterial

Mutation

Codon Optimized for bacterial expression, T7 gene…

Promoter

T7

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-ACTB-C200-T7

Plasmid

#229850

Purpose

Donor vector for the ACTB site containing T7 sequence for detection of HR products.

Depositor

Natsuko Chiba

Insert

Partial sequence of ACTB gene

Use

Hr donor vector for human actb gene.

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-T7-PAB1-NLuc

Plasmid

#219984

Purpose

PAB1-5'UTR-NLuc mRNA synthesis

Depositor

Simon Alberti

Insert

PAB1-5'UTR-NLuc (PAB1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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T7-10-QUAS-GFP_ColE1

Plasmid

#171657

Purpose

QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-10-QUAS

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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