We narrowed to 11,678 results for: cel.2

1. pLsCas13a-gRNA-2

   Plasmid

   #164866

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS_KS_attB2_SA(2)_T2AGeneSwitch

   Plasmid

   #125216

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GeneSwitch
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS_KS_attB2_SA(2)_T2AGAL80

   Plasmid

   #125219

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL80
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pegRNA_LMNA_CR-2-5

   Plasmid

   #199275

   Purpose

   pegRNA used to correct LMNA (c.1824C > T) mutation

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   LMNA 1824TtoC pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-sgAAVS1-2

   Plasmid

   #129727

   Purpose

   Expressing SpCas9 and sgAAVS1-2

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-2
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-SREBP-2

   Plasmid

   #204200

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Use

   Tags

   T7x3

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYU-2-Env

   Plasmid

   #133997

   Purpose

   Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

   Depositor

   Himanshu Garg

   Article

   Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

   Insert

   Envelope Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA 2 GAPDH

   Plasmid

   #83809

   Purpose

   Express Sg-2 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiTE(2)-Gal4

   Plasmid

   #220451

   Purpose

   Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiTE(2)-Gal4DBD

    Plasmid

    #220454

    Purpose

    Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4DBD-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiTE(2)-LexAGAD

    Plasmid

    #220460

    Purpose

    Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-LexAGAD-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiTE(2)-p65AD

    Plasmid

    #220457

    Purpose

    Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-p65AD-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psiT-GEM(2)

    Plasmid

    #220448

    Purpose

    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A-T#2

    Plasmid

    #171516

    Purpose

    deletion of a genomic locus in T(Brachyury) gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    T (T Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuide-RRBP1-2

    Plasmid

    #92157

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MacroH2A.2-c002

    Plasmid

    #173089

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A2 (MACROH2A2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K167-K372

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. hNedd4-2 CS

    Plasmid

    #86041

    Purpose

    full length NeddL3 with C to S mutation with His Xpress tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

    Insert

    hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    C942S mutation in HECT domain

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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