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pBx1-VHH template3-3XMyc-Spacer

Plasmid

#176210

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template3-3XMyc-Spacer

Use

Tags

Myc tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Use

Tags

Myc tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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(365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

Plasmid

#168188

Purpose

HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

Plasmid

#168191

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nLag6-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcNB2

Plasmid

#89242

Purpose

Resurfaced Cell-penetrating 5F7 nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB2 (5F7)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcNB3

Plasmid

#89243

Purpose

Resurfaced Cell-penetrating B-lac nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB3 (B-Lac)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

Plasmid

#168190

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nLag6-(EAAAK)4-OGA(544-706)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP955

Plasmid

#104164

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1172

Plasmid

#104161

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1005

Plasmid

#104160

Purpose

Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP1174

Plasmid

#104162

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

Plasmid

#163084

Purpose

rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

anti-GFP nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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