We narrowed to 886 results for: nanobody

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pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

Plasmid

#163084

Purpose

rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

anti-GFP nanobody

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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cDNA5-FRT/TO-APT2-LAMA-F98

Plasmid

#234427

Purpose

Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98 (LYPLA2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-APT2-LAMA-G97

Plasmid

#234426

Purpose

Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97 (LYPLA2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110

Plasmid

#135480

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110.i1

Plasmid

#135481

Purpose

E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110.i1

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH72

Plasmid

#202467

Purpose

Expresses the nanobody VHH72 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-LaM4-EGFP

Plasmid

#182641

Purpose

Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, EGFP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-VCAM1

Plasmid

#202471

Purpose

Expresses the nanobody VCAM1 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VCAM1

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET-15b-aδNb107

Plasmid

#220319

Purpose

Expresses the anti-IgD nanobody 107 (aδNb107) in the bacterial periplasm. aδNb107 binds to human IgD.

Depositor

James McDonnell

Article

Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

Insert

aδNb107

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-15b-aδNb367

Plasmid

#220320

Purpose

Expresses the anti-IgD nanobody 367 (aδNb367) in the bacterial periplasm. aδNb367 binds to human IgD.

Depositor

James McDonnell

Article

Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

Insert

aδNb367

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-15b-aδNb408

Plasmid

#220321

Purpose

Expresses the anti-IgD nanobody 408 (aδNb408) in the bacterial periplasm. aδNb408 binds to the Cδ2 domain of human IgD.

Depositor

James McDonnell

Article

Vester et al Sci Rep. 2025 Jul 8;15(1):24455. doi: 10

Insert

aδNb408

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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