We narrowed to 219 results for: ube2s

1. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Use

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. UbcH5A

   Plasmid

   #61081

   Purpose

   express a human E2

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   UbcH5A (UBE2D1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-myc-MmUbc7/2776

   Plasmid

   #11488

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc7 (Ube2g2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCI-Neo-HA-MmUbc6/2774

   Plasmid

   #11482

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc6 (Ube2j2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-myc-MmUbc7mut/2777

   Plasmid

   #11489

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc7 (Ube2g2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   cys 889 mutated to ser

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-KG-MmUbc7/2783

   Plasmid

   #11490

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc7 (Ube2g2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Promoter

   Available since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-KG-MmUbc6/2790

   Plasmid

   #11491

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc6 (Ube2j2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Promoter

   Available since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCMV3flag8HOIP

   Plasmid

   #50015

   Purpose

   Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   ring finger protein 31 (RNF31 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-OTUB1 WT-pcDNA3.1

    Plasmid

    #118209

    Purpose

    Expresses FLAG-OTUB1 WT in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-Neo-HA-MmUbc6 mut/2775

    Plasmid

    #11483

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc6 (Ube2j2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cys 94 mutated to ser

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-6P-1-Cys-HA-Ubiquitin

    Plasmid

    #185356

    Purpose

    In vitro ubiquitination assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Ubiquitin
    

    Use

    Tags

    GST, HA(x2)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-OTUB1 C91S-pcDNA3.1

    Plasmid

    #118210

    Purpose

    Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-OTUB1 T134R-pcDNA3.1

    Plasmid

    #118211

    Purpose

    Expresses FLAG-OTUB1 T134R mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 134 to arginine

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-E2

    Plasmid

    #175242

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-C1-G2BRSCR

    Plasmid

    #185345

    Purpose

    Expression of soluble G2BR with scrambled sequence

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    scrambled gp78 G2BR sequence
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    scrambled sequence

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-NHK-MYC-His6

    Plasmid

    #185350

    Purpose

    Assessing of ubiquitination in cells

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    a1-antitrypsin Hong Kong Null mutant
    

    Use

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    NHK mutant

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR3-FLAG-Ubc13-WT

    Plasmid

    #43938

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR3-FLAG-Ubc13-C87A

    Plasmid

    #43939

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    C87A

    Promoter

    Available since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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