We narrowed to 1,186 results for: MS2;

1. pHR SFFVp MCP-mCherry IRES h2b-diRFP

   Plasmid

   #102351

   Purpose

   Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.

   Depositor

   Jared Toettcher

   Article

   Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

   Insert

   MS2 Coat Protein
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NLS-HA-2xMCP-KRAB

   Plasmid

   #126589

   Purpose

   Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   2XMCP-KRAB
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD442

   Plasmid

   #153024

   Purpose

   dCas9, MCP-SoxS(R93A/S101A)

   Depositor

   Jesse Zalatan

   Article

   Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

   Insert

   dCas9, MCP-SoxS(R93A/S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Available since

   Aug. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC NLS HA stdMCP Halo

   Plasmid

   #104098

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdMCP-Halo
   

   Use

   Lentiviral

   Tags

   C-terminal Halo-tag, N-terminal NLS, and Two syno…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage UbiC NLS HA stdMCP stdHalo

   Plasmid

   #104999

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   stdMCP-stdHalo
   

   Use

   Lentiviral

   Tags

   HA tag, NLS, Two synonymous copies of the Halo-ta…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-dzCas9-Act3.0

   Plasmid

   #158414

   Purpose

   CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154786

   Purpose

   Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Synthetic, Fly)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and mCherry

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-dSpRY-Act3.0

   Plasmid

   #158416

   Purpose

   CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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