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Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254PromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7a
Plasmid#109655PurposeMuscle specific zebrafish expression of mKate2 fused to rab7aDepositorInsertrab7a (rab7a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7b
Plasmid#109656PurposeMuscle specific zebrafish expression of mKate2 fused to rab7bDepositorInsertrab7b (rab7b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
RP78 pUAST Orco mRNA
Plasmid#53431PurposeExpresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS controlDepositorInsertOrco (Orco )
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8a
Plasmid#109657PurposeMuscle specific zebrafish expression of mKate2 fused to rab8aDepositorInsertrab8a (rab8a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-33-MC_hammer
Plasmid#138989PurposeIVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST myc MOV10 K530A
Plasmid#51719Purposeplasmid expresses myc-tagged MOV10 K530ADepositorInsertMOV10 K530A (MOV10 Human)
UseTagsmycExpressionMammalianMutationK530APromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK IRF9 3'UTR
Plasmid#19863DepositorInsertIRF9 3'UTR (IRF9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
KG802: pMVP (L3-L2) V5 epitope tag + polyA
Plasmid#121755PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertV5 epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN402: pMVP (R4-R3) Firefly Luciferase
Plasmid#121741PurposepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertFirefly Luciferase
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KW701: pMVP (L5-L4) AID
Plasmid#121723PurposepMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.DepositorInsertAID (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
II901: pMVP (L5-L4) FKBP DD
Plasmid#121720PurposepMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST myc MOV10 D645N
Plasmid#51720Purposeplasmid expresses myc-tagged MOV10 D645NDepositorInsertMOV10 D645N (MOV10 Human)
UseTagsmycExpressionMammalianMutationD645NPromoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_MBD1_iso1_2
Plasmid#86304PurposeEncodes gRNA for 3' target of human MBD1_iso1DepositorInsertgRNA against MBD1_iso1 (MBD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD2A_2
Plasmid#86334PurposeEncodes gRNA for 3' target of human GATAD2ADepositorInsertgRNA against GATAD2A (GATAD2A Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD2A_1
Plasmid#86333PurposeEncodes gRNA for 3' target of human GATAD2ADepositorInsertgRNA against GATAD2A (GATAD2A Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
UseTagsExpressionBacterialMutationPromoterrbcS-T5: T7, chaperones: T11Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
UseTagsExpressionBacterialMutationPromoterrbcS-T1: T7, chaperones: T8Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS5R1AtR2NtB2
Plasmid#160884PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S5
UseTagsExpressionBacterialMutationPromoterrbcS-S5: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS2R1AtR2NtB2
Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S2: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA27
Plasmid#158505PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
II801: pMVP (L5-L4) ecDHFR DD
Plasmid#121721PurposepMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.DepositorInsertecDHFR Degron Domain (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TU#1806_CRISPR_unc-73_exon21
Plasmid#82360Purposeto create unc-73E null alleleDepositorInsertCas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
KJ801: pMVP/Hygro-DEST
Plasmid#121858PurposepMVP destination vector, empty expression vector backbone w/ Hygro selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dnm2b-DN
Plasmid#109574PurposeMultisite gateway vector for 3' tagging with dominant negative Dynamin2bDepositorInsertDynamin2b (dnm2b Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-EHD1b-dEH
Plasmid#109567PurposeMultisite gateway vector for 3' tagging with EHD1b-delta-EHDepositorInsertEHD1b (ehd1b Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdelta-EHPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB801: pMVP/PB/eCFP-DEST
Plasmid#121872PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_MBD1_iso1_1
Plasmid#86303PurposeEncodes gRNA for 3' target of human MBD1_iso1DepositorInsertgRNA against MBD1_iso1 (MBD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
KU701: pMVP (L5-L4) eGFP
Plasmid#121707PurposepMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.DepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF652
Plasmid#86270PurposeDonor vector for 3' FLAG tag of human ZNF652DepositorInsertZNF652 homology arms (ZNF652 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
IO901: pMVP (L5-L4) BioID2
Plasmid#121724PurposepMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.DepositorInsertBioID2 (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionTagsExpressionInsectMutationPromoterAedes aegypti poly-ubiquitinAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
JL302: pMVP/Puro-DEST
Plasmid#121856PurposepMVP destination vector, empty expression vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK c2orf42 3'UTR
Plasmid#19862DepositorInsertc2orf42 3'UTR (C2orf42 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only