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pMC-dest-BsaI-cmlc2:mCherry

Plasmid

#241213

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SA-EGFP-pA

Plasmid

#241211

Purpose

Middle entry vector for mini-Golden subcloning platform; eGFP

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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memb-mNeongreen

Plasmid

#241175

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA

Plasmid

#200542

Purpose

mini circle middle entry plasmid for CreER-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_mCherry-pA

Plasmid

#200521

Purpose

Mini circle middle entry plasmid for mCherry- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-mCherry-pA

Plasmid

#200528

Purpose

mini circle middle entry plasmid for membrane mCherry-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_BFP-pA

Plasmid

#200533

Purpose

mini circle middle entry plasmid for BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-BFP-pA

Plasmid

#200537

Purpose

mini circle middle entry plasmid for membrane BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_EGFP-pA

Plasmid

#200513

Purpose

Mini circle middle entry plasmid for GFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP-pA

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-RYBP NZF (20-51)

Plasmid

#235652

Purpose

Bacterial expression of human RYBP NZF (20-51)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

RYBP NZF (RYBP Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 20-51 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG014

Plasmid

#231335

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG003

Plasmid

#231330

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM211 His-Ubp6

Plasmid

#226359

Purpose

Expression of yeast Ubp6

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

ubp6 (UBP6 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP-NLS

Plasmid

#222604

Purpose

Expression of MAC3-tagged GFP-NLS (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC3-N-GFP-NES

Plasmid

#222606

Purpose

Expression of MAC3-tagged GFP-NES (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-NTRK3

Plasmid

#222612

Purpose

Expression of MAC3-tagged NTRK3

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

NTRK3 (NTRK3 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC3-N-GFP-NLS

Plasmid

#222607

Purpose

Expression of MAC3-tagged GFP-NLS (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear localizaition sequence (NLS)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC3-C-GFP-NES

Plasmid

#222603

Purpose

Expression of MAC3-tagged GFP-NES (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC3-N-GFP-Myr

Plasmid

#222608

Purpose

Expression of MAC3-tagged GFP-Myr (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC3-C-GFP-Myr

Plasmid

#222605

Purpose

Expression of MAC3-tagged GFP-Myr (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSCL194

Plasmid

#222225

Purpose

Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 1/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL195

Plasmid

#222226

Purpose

Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 2/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL368

Plasmid

#222227

Purpose

Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 3/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SNAPf-BASU-His

Plasmid

#218450

Purpose

E coli expression vector for SNAPf-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAPf-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SLC25A19

Plasmid

#166209

Purpose

Gateway entry vector for N. furzeri SLC25A19

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SLC25A19

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SMPDL3B

Plasmid

#166211

Purpose

Gateway entry vector for N. furzeri SMPDL3B

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SMPDL3B

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SNRNP70

Plasmid

#166212

Purpose

Gateway entry vector for N. furzeri SNRNP70

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SNRNP70

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SOD2(v1)

Plasmid

#166214

Purpose

Gateway entry vector for N. furzeri SOD2

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SOD2(v1)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SOD2(v2)

Plasmid

#166215

Purpose

Gateway entry vector for N. furzeri SOD2

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SOD2(v2)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-STARD10

Plasmid

#166216

Purpose

Gateway entry vector for N. furzeri STARD10

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

STARD10

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-TM9SF3

Plasmid

#166218

Purpose

Gateway entry vector for N. furzeri TM9SF3

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

TM9SF3

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-TXNL1(v1)

Plasmid

#166219

Purpose

Gateway entry vector for N. furzeri TXNL1

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

TXNL1(v1)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PDHB

Plasmid

#166195

Purpose

Gateway entry vector for N. furzeri PDHB

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PDHB

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PNPT1

Plasmid

#166196

Purpose

Gateway entry vector for N. furzeri PNPT1

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PNPT1

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PRDX1

Plasmid

#166197

Purpose

Gateway entry vector for N. furzeri PRDX1

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PRDX1

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PRDX4

Plasmid

#166198

Purpose

Gateway entry vector for N. furzeri PRDX4

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PRDX4

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PRPF39(1of2)

Plasmid

#166199

Purpose

Gateway entry vector for N. furzeri PRPF39

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PRPF39(1of2)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PSMA3

Plasmid

#166200

Purpose

Gateway entry vector for N. furzeri PSMA3

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PSMA3

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-PSMD11

Plasmid

#166201

Purpose

Gateway entry vector for N. furzeri PSMD11

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

PSMD11-LOC107387616

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-RPL30

Plasmid

#166203

Purpose

Gateway entry vector for N. furzeri PRL30

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

RPL30

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-RPS10

Plasmid

#166204

Purpose

Gateway entry vector for N. furzeri RPS10

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

RPS10

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-RPS2

Plasmid

#166205

Purpose

Gateway entry vector for N. furzeri RPS2

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

RPS2

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SCARB2(v1)

Plasmid

#166206

Purpose

Gateway entry vector for N. furzeri SCARB2

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SCARB2(v1)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SCARB2(v2)

Plasmid

#166207

Purpose

Gateway entry vector for N. furzeri SCARB2

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SCARB2(v2)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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