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Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-9
Plasmid#228993PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-9
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-7
Plasmid#228997PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-8
Plasmid#228992PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-1
Plasmid#228995PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-5
Plasmid#228991PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-5
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1b
Plasmid#194292PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJDepositorInsertcavin1b (cavin1b Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVL90 - VND7
Plasmid#116035Purposeentry vector for GreenGate cloning method, CDSDepositorInsertVND7 (VND7 Mustard Weed)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon oG
Plasmid#131778PurposeINTRSECT 2.0DepositorInsertrabies glycoprotein - oG
UseAAV; IntrsectTagsnoneExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMYH9-WT-V5
Plasmid#183512PurposeGateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.DepositorInsertMYH9 (MYH9 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV INS2x mRFP-3MREs (AAV-beta)
Plasmid#206928PurposeBeta cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterINSx2Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 TOMM20
Plasmid#157761PurposeExpression of TOMM20:mcherry in cellsDepositorInsertTOMM20 (TOMM20 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-Cas9n-Act3.0
Plasmid#178956PurposeIt consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_Halo_GluA2
Plasmid#170454PurposeExpresses halo tagged GluA2 in neuronal cellsDepositorInsertGluA2 (Gria2 Mouse)
UseLentiviralTagsHaloExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-SpRYn-Act3.0
Plasmid#178959PurposeIt consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-F1
Plasmid#194592PurposeBacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_SNAP_NR1
Plasmid#170458PurposeExpresses SNAP tagged NR1 in neuronal cellsDepositorInsertNR1 (Grin1 Rat)
UseLentiviralTagsSNAPExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-E2
Plasmid#194590PurposeBacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertE2 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry C1 MFF
Plasmid#157760PurposeExpression of mcherry:MFF in cellsDepositorInsertMFF (MFF Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_-CMV-Pcdh15_mini_V4-CD2
Plasmid#199184PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.DepositorInsertmmPcdh15_mini_V4-CD2
UseAAVTagsExpressionMutationEC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only