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pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Publication

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_Gq_dTomato

Plasmid

#213817

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_Gq_dTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_GCaMP6f

Plasmid

#213917

Purpose

AAV construct expressing GCaMP6f driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe3_Gq_dTomato

Plasmid

#213942

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_C1V1_eYFP

Plasmid

#213831

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_ChR2_mCherry

Plasmid

#213915

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_C1V1_eYFP

Plasmid

#213816

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFP

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo

Plasmid

#174378

Purpose

also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Has service

AAV Retrograde

Insert

FLPo recombinase

Use

AAV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_BiPVe4_ChRmine_mScarlet

Plasmid

#255996

Purpose

ChRmine opsin driven by PV chandelier cell-targeting enhancer BiPVe4

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

ChRmine-mScarlet

Use

AAV

Available since

Aug. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_Gq_dTomato

Plasmid

#213916

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe3_ChRmine_mScarlet

Plasmid

#255994

Purpose

ChRmine opsin driven by PV basket cell-targeting enhancer BiPVe3

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

ChRmine-mScarlet

Use

AAV

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_S5E2_ChRmine_mScarlet

Plasmid

#255993

Purpose

ChRmine opsin driven by PV-targeting enhancer S5E2

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

ChRmine-mScarlet

Use

AAV

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1929

Plasmid

#205968

Purpose

JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH crRNA

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHS1477

Plasmid

#205970

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH all proteins, with His-tagged Cas11

Tags

14xHis on Cas11

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1232

Plasmid

#205969

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1801

Plasmid

#205966

Purpose

JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1871

Plasmid

#205976

Purpose

U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas8-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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