We narrowed to 2,414 results for: organization Addgene

1. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO_eGFP-pScaC-MTS

   Plasmid

   #228827

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   MTS and eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACEBac1_Strep-Psc-BICD2

   Plasmid

   #228829

   Purpose

   Insect cell expression of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ClhN-p500-Erg6-V5-APEX2-URA

   Plasmid

   #207070

   Purpose

   Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Erg6
   

   Use

   Tags

   V5-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pERG6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ClhN-p1k-OM14-V5-APEX2-Ura

   Plasmid

   #207073

   Purpose

   Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   OM14
   

   Use

   Tags

   V5-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pOM14

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWB602-po-mCerulean

   Plasmid

   #213861

   Purpose

   Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

   Insert

   Peroxisome transit peptide fused mCerulean
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ClhN-p1k-Cox4-V5-APEX2-Ura

   Plasmid

   #207061

   Purpose

   Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Cox4
   

   Use

   Tags

   V5-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pCOX4

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ClhN-p1k-Cox4-GFP-APEX2-Ura

   Plasmid

   #207062

   Purpose

   Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Cox4
   

   Use

   Tags

   GFP-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pCOX4

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ClhN-pERG6-Pex3-4V5-APEX2-URA

   Plasmid

   #207068

   Purpose

   Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Pex3
   

   Use

   Tags

   4xV5-APEX2

   Expression

   Yeast

   Mutation

   Promoter

   pERG6

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ClhN-pLSP1-Pex3-4V5-APEX2-URA

    Plasmid

    #207069

    Purpose

    Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pLSP1

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ClhN-pTPI1-Emc1-GFP-APEX2-Ura

    Plasmid

    #207074

    Purpose

    Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Emc1
    

    Use

    Tags

    GFP-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ClhN-pTPI1-Cox4-GFP-APEX2-Ura

    Plasmid

    #207075

    Purpose

    Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Cox4
    

    Use

    Tags

    GFP-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWT35S-AtTIM21(1-50aa)-mCherry

    Plasmid

    #194047

    Purpose

    Transient expression of AtTIM21-mCherry in plant cell (Mitochondria)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Use

    Tags

    AtTIM21 1–50aa

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H2B-HT-emiRFP703

    Plasmid

    #183985

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    HaloTag, emiRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H2B-HT-mGold

    Plasmid

    #183988

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    HaloTag, mGold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-Dnase1l1l-mRFP

    Plasmid

    #169002

    Purpose

    Express zebrafish Dnase1l1l-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Dnase1l1l-mRFP (dnase1l3l Zebrafish)
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_V577D-talin1 (1-2541)

    Plasmid

    #166115

    Purpose

    expresses talin-1-V577D in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    V577D-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP_P229L-talin1 (1-2541)

    Plasmid

    #166113

    Purpose

    expresses talin-1-P229L in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    P229L-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFE-sfGFP

    Plasmid

    #132998

    Purpose

    pFE carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFE-dabA2

    Plasmid

    #133001

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge