We narrowed to 9,915 results for: pET

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pET-6xHis-GFP(-6)-H2_mCherry(-6)

Plasmid

#205049

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Publication

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)

Tags

MGHHHHHGGA

Expression

Bacterial

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mStrawberry-15b

Plasmid

#206868

Purpose

Express tTALE-mStrawberry

Depositor

Kyubong Jo

Publication

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mStrawberry

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-eSpCas9-NLS-6xHis

Plasmid

#207383

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, amino acids 1005-1013 repl…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-U

Plasmid

#190969

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Publication

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-U

Plasmid

#190973

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Publication

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-C322A

Plasmid

#154191

Purpose

To express LanCL1-C322A in bacterial cells

Depositor

Wilfred van der Donk

Publication

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys322->Ala322

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Publication

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Tags

His6-MBP

Expression

Bacterial

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SUMO mCER-Prx2

Plasmid

#158226

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

Depositor

Tobias Dick

Publication

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Bacterial

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a RUVBL2 Delete

Plasmid

#159143

Purpose

Expression and protien purification of a mutated RUVBL2

Depositor

Eric Erquan Zhang

Publication

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Mutation

amino acid 2-456, Thr127-Glu233 mutated to GPPG

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PET.SUMO eIF4G1 +MIC 1-200

Plasmid

#158792

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

Depositor

Benjamin Blencowe

Publication

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon) codon optimized

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK jannaschii

Plasmid

#108948

Purpose

heterologous expression of MVK of M. jannaschii in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-CH2 (1192-1318)

Plasmid

#99338

Purpose

Bacterial expression of mouse CBP CH2 domain

Depositor

Peter Wright

Publication

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

CBP CH2 (1192-1318) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Publication

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Publication

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET-21a(+) SurE D9A

Plasmid

#12686

Depositor

SeWon Suh

Publication

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 9 to Alanine

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
Rv2002 M3/pET-28b(+)N-tag

Plasmid

#13057

Depositor

SeWon Suh

Publication

Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

Insert

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Tags

His

Expression

Bacterial

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha tentacle delete CP

Plasmid

#13299

Depositor

John Cooper

Publication

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)

Expression

Bacterial

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only