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MTK234_029

Plasmid

#123909

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hThal5.10-2_hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 C539A

Plasmid

#133008

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

C539A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 H524A

Plasmid

#133007

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

H524A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 D353A

Plasmid

#133006

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

D353A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJB557

Plasmid

#115967

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJB554

Plasmid

#115964

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW101

Plasmid

#115944

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW098

Plasmid

#115941

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW097

Plasmid

#115940

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP 2x K-turn

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW095

Plasmid

#115938

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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