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pEE057

Plasmid

#176814

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1386-1535_S

Plasmid

#147540

Purpose

Mammalian Expression of HsNot1iso1_1386-1535

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1386-1535 (CNOT1 Human)

Expression

Mammalian

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-As

Plasmid

#138097

Purpose

Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-As

Plasmid

#138100

Purpose

Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1408

Plasmid

#99032

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1928

Plasmid

#99033

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8e-Cas9NG

Plasmid

#203328

Purpose

Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

ABE8e-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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