Addgene

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h3.7SP-Crtta

Plasmid

#20180

Depositor

Jeffrey Whitsett

Article

Tichelaar et al J Biol Chem. 2000 Apr 21. 275(16):11858-

Insert

h3.7SP-Crtta (SFTPC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Ph

Plasmid

#46818

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pG5ElbLuc

Plasmid

#89341

Purpose

The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.

Depositor

Roger Davis

Article

Seth et al J Biol Chem. 1992 Dec 5;267(34):24796-80

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAW12

Plasmid

#110224

Purpose

integration of ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCY 3090-06

Plasmid

#36233

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

URA3

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-URA3

Expression

Mutation

Promoter

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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PCLP-2/NT-IgG (104)

Plasmid

#24423

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Use

Tags

Human IgG1 Fc

Expression

Mammalian

Mutation

N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCY 3040-05

Plasmid

#36220

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

LEU2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEGFP-LEU2

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3080-04

Plasmid

#36228

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-TRP1

Expression

Mutation

Promoter

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3080-06

Plasmid

#36230

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

URA3

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-URA3

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3040-04

Plasmid

#36219

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEGFP-TRP1

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3120-04

Plasmid

#36238

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-13Myc-TRP1

Expression

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Use

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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nSYB-GAL80-DD

Plasmid

#124778

Purpose

Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GAL80 (GAL80-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

nSynaptobrevin

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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CC10-rtTA

Plasmid

#20179

Depositor

Jeffrey Whitsett

Article

Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-

Insert

rcc10 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
10XUAS-flp-DD

Plasmid

#124777

Purpose

Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized FLP (FLP-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCY 3030-02

Plasmid

#36215

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEmCFP

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCFP-hphMX4

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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