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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3

Plasmid

#203498

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B-EGFP

Plasmid

#210366

Purpose

Expresses TMEM106B fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-BRD4 BD1

Plasmid

#196544

Purpose

Expression of BRD4 Bromodomain (BD1) in E.coli

Depositor

Byung Il Lee

Article

Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

Insert

BRD4 BD1 (BRD4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pSW454 - pUFO

Plasmid

#115978

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pUFO

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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HHM-iATPSnFR1.1

Plasmid

#102547

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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