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TRCN0000072254
Plasmid#78161PurposeLuciferase controlDepositorInsertLuciferase
UseRNAiTagsExpressionMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-v2_SigP:NLuc
Plasmid#197268PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-v2 homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P2 Promoter-FLuc
Plasmid#154267PurposeFirefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.DepositorInsertIGF-1 P2 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P2 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000231737
Plasmid#78162PurposeLuciferase controlDepositorInsertLuciferase
UseRNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + WT miR-181 BS in CDX2 3UTR
Plasmid#22792DepositorInsertWild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in CDX2 3UTR
Plasmid#22793DepositorInsertMutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT&Mut-SM
Plasmid#215916PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT-SM
Plasmid#215917PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut1-SM
Plasmid#215918PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut2-SM
Plasmid#215919PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT&Mut-CM
Plasmid#215920PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmMRE11
Plasmid#208384PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.DepositorInsertsgMRE11 (Mre11a Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3
Plasmid#208389PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.DepositorInsertsgZBP1 (Zbp1 Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmMLKL
Plasmid#208391PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.DepositorInsertsgMLKL (Mlkl Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_SigP:NLuc
Plasmid#197263PurposeCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 732
Plasmid#194195PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 732)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 884
Plasmid#194196PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 884)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449
Plasmid#194197PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 449)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_SMAD-FOS_Mutant
Plasmid#194188PurposeIncludes the promoter (1kb) of SMTS with mutated SMAD/FOS binding siteDepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated SMAD/FOS Site, Region 3-15nt of 1000ntPromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_STAT1-2_Mutant
Plasmid#194189PurposeIncludes the promoter (1kb) of SMTS with mutated STAT1/2 binding siteDepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated STAT1/2 site, Region 653-673nt of 1000ntPromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)
Plasmid#194191PurposeIncludes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation4 mutated KLF binding sites (positions 449,548,73…PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)
Plasmid#194192PurposeIncludes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation3 mutated KLF binding sites (positions 449,548,73…PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 548
Plasmid#194194PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 548)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73040PurposeExpress Nluc and copGFP dual reporter in mammalian cellsDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterPGK (phosphoglycerate kinase 1)Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGPDluxAB(Ura)
Plasmid#1119DepositorInsertbacterial luciferase
UseTagsExpressionYeastMutationn/aPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pAKgfplux1
Plasmid#14083DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73039PurposeExpress Nluc, copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancer and chicken bet…Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAKlux1
Plasmid#14073DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAKlux2.1
Plasmid#14081DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT-TK
Plasmid#31549DepositorInsertLoxP
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGAL - FFL[SEL]
Plasmid#1102DepositorInsertfirefly luciferase
UseTagsExpressionYeastMutationn/aPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAKlux1.1
Plasmid#14074DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
G12-CASE
Plasmid#190714PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagsNluc is inserted at A133/F134 within GNA12 and cp…ExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P1 Promoter-FLuc
Plasmid#154266PurposeFirefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.DepositorInsertIGF-1 canonical P1 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P1 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoB
Plasmid#196052PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-hACE2
Plasmid#173431Purposeprotein expression plasmid of LgBiT-hACE2-IgG1 FcDepositorInsertLgBiT-hACE2-IgG1 Fc (ACE2 Human)
UseLuciferaseTagsIgG1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmcGAS
Plasmid#208385PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.DepositorInsertsgcGAS (Cgas Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmSTING
Plasmid#208386PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.DepositorInsertsgSTING (Sting1 Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3E-hGPR56 e1m promoter normal
Plasmid#52298PurposeReporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.DepositorInsertADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)
Plasmid#229771Purpose3xFlag-tagged human Col4A4 to be used with N- or C-tagged LgBiT Col4A5 and SmBiT Col4A3 in Nanobit assay systemDepositorInsertHuman collagen type IV alpha 4 chain (COL4A4) (COL4A4 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmATM
Plasmid#208392PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.DepositorInsertsgATM (Atm Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P1 Promoter-FLuc2P
Plasmid#154261PurposeDestabilized firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.DepositorInsertIGF-1 canonical P1 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P1 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P2 Promoter-FLuc2P
Plasmid#154262PurposeDestabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.DepositorInsertIGF-1 P2 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P2 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only
