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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC22A3

Plasmid

#132024

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A3 (SLC22A3 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
Split 'n coexpress CUL3-RBX1

Plasmid

#52293

Purpose

bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Inserts

Cul3 N-terminal domain (CUL3 Human)
Cul3 C-terminal domain (CUL3 Human)
RBX1 (Rbx1 Mouse)

Tags

GST, IRES, and TEV

Expression

Bacterial

Mutation

contains aa 1-309, contains aa 310-768, and conta…

Promoter

IRES and tac

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
MOS

Plasmid

#64120

Purpose

A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

OCT4-2A-SOX2 (POU5F1, SOX2 Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2K

Plasmid

#20925

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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SARS-CoV2 Nucleocapsid

Plasmid

#153201

Purpose

Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

Depositor

Peter Klein

Article

Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))

Tags

Myc tag (5X)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEP4 E02S EN2L

Plasmid

#20922

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMminiSOX

Plasmid

#206383

Purpose

Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Mouse, Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-MAPPER

Plasmid

#117721

Purpose

MAPPER Selectively Labels ER-PM Junctions

Depositor

Jen Liou

Article

Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

Insert

MAPPER

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-mGFP

Plasmid

#34831

Depositor

Esteban Dell'Angelica

Article

Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

Insert

LAMP1 (LAMP1 Human)

Tags

GSTGSTGSTGA linker, Kozak, and mGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-MAC251-vpx

Plasmid

#115842

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense

Plasmid

#46834

Purpose

Express cGFP transcript ending in the wildtype MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pscALPS gag-gfp/vpx

Plasmid

#115808

Purpose

SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS gag-gfp/deltavpx

Plasmid

#115807

Purpose

SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PSG

Plasmid

#59731

Purpose

Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

TJP1 (TJP1 Human)

Tags

HIS and TEV

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pscALPS gag-gfp Blasti

Plasmid

#115803

Purpose

Express gag-gfp fusion driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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