We narrowed to 2,365 results for: DAP

1. pCas4_1_Δ2_VA

   Plasmid

   #186439

   Purpose

   Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCasΔ4_1_2_VA

   Plasmid

   #186440

   Purpose

   Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas-mut4_1_2_VA

   Plasmid

   #186441

   Purpose

   Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas-4/1_2_VB

   Plasmid

   #186442

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas-4/1Δ2_VB

   Plasmid

   #186443

   Purpose

   Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCas12(PI)

   Plasmid

   #186448

   Purpose

   Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K613A, K617A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas12b

   Plasmid

   #186449

   Purpose

   Encodes Cas12b from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas12b(PI)

   Plasmid

   #186451

   Purpose

   Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R122A, G143P

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACT2-μ1B (137-423)

   Plasmid

   #198172

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1B (137-423)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBT9-AAGAB

    Plasmid

    #198332

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.GFP-KIF3A tail 419-702

    Plasmid

    #193923

    Purpose

    Mammalian expression of GFP-tagged Kif 3A tail 419-702

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3A (KIF3A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR2_+10D+7U

    Plasmid

    #149393

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    7bp inserted upstream, and 10bp inserted downstre…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFL GST-UBXN2A

    Plasmid

    #169016

    Purpose

    Expresses GST-UBXN2A (human) in insect cells

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMKO.1-shNuf2

    Plasmid

    #160961

    Purpose

    Nuf2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nuf2 shRNA (Nuf2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EcRfaHKOW-L1L2NusG

    Plasmid

    #113126

    Purpose

    Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107, replaced L1 and L2 loops …

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28P_dCrk

    Plasmid

    #131145

    Purpose

    Expresses 6XHis::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-biotin-myc- mCE

    Plasmid

    #82475

    Purpose

    Expresses mRNA capping enzyme containing N terminal biotin and myc tag

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Biotin (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF-HA-TSAd-Y89XF

    Plasmid

    #84559

    Purpose

    mammalian expression of TSAd-Y89XF

    Depositor

    Anne Spurkland

    Article

    Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y279F, Y290F, Y305F

    Promoter

    EF1a

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only