Addgene

Showing: 1421 - 1440 of 14941 results

Results per page:

Sort results by:

Phat2-dr-Alox12-PLAT-EGFP-6H

Plasmid

#187190

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000069463 (alox12 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Phat2-hs-PKCgamma-C2-EGFP-6H

Plasmid

#187115

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000126583 (PRKCG Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RVD-hC9

Plasmid

#173319

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

HD half RVD at Position9

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RVD-AG9

Plasmid

#173268

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NN dimer RVD at Position9

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lentiguide-Blast-eNMU710

Plasmid

#172658

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-eNMUA/pegRNA-eNMUB

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-HD252

Plasmid

#172656

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD252A/pegRNA-HD252B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGH50

Plasmid

#107254

Purpose

Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp-fkbp12

Use

Suicide vector for destined for double homologous…

Tags

GFP and FKBP12

Expression

Bacterial

Mutation

Promoter

native

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
P0050::kaiBC S431A T432A NS1

Plasmid

#101837

Purpose

drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431A T432A with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431A T432A

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
P0050::kaiBC S431E T432E NS1

Plasmid

#101836

Purpose

drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431E T432E with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431E T432E

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKtheoRaj12

Plasmid

#69942

Purpose

pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj12,cisRaj12,sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKtheoRaj11

Plasmid

#69939

Purpose

pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj11,cisRaj11,sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKbreak12

Plasmid

#69938

Purpose

pSTC2,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

CisRaj12-sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_(GLuc)_INT[S1Apt]

Plasmid

#68425

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct with S1 streptavidin aptamer

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-3XMyc-TRAF2 S408A

Plasmid

#44109

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S408A (TRAF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 408 to Alanine

Promoter

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
pWB-TRAF2 S102A

Plasmid

#44135

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Serine 102 to Alanine

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S102A

Plasmid

#44106

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 102 to Alanine

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pCMV myc DAF-12(P687S)

Plasmid

#32567

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12(P687S) (daf-12 Nematode)

Use

Tags

myc epitodpe

Expression

Mammalian

Mutation

Proline 687 to Serine

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge