We narrowed to 19,332 results for: gateway

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Publication

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E3

Plasmid

#15789

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONRp5-p2 SspB-mCherry-CShroom3

Plasmid

#170977

Purpose

C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Publication

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

CShroom3 (Shroom3 Mouse)

Use

Gateway Cloning

Tags

SspB and mCherry

Mutation

Isoform X2, deleted aminoacids 1 - 1388

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166014

Purpose

mammalian expression of HCoV-HKU1 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-HKU1 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pInducer20 EGFP-TEADi

Plasmid

#140145

Purpose

Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

Depositor

Ramiro Iglesias-Bartolome

Publication

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Publication

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pYPQ141-ZmUbi-RZ-As

Plasmid

#86196

Purpose

AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1 gRNA cloning site for ribozyme cleavage

Use

CRISPR

Tags

Hammerhead ribozyme and HDV ribozyme

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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All_in_one_CRISPR/Cas9_LacZ

Plasmid

#74293

Purpose

"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

Depositor

Lynne Postovit

Publication

Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

Inserts

LacZ-alpha
Cas9
mCherry

Use

CRISPR

Tags

HA

Expression

Bacterial and Mammalian

Promoter

Lac promoter, SV40, and T7 promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-BRPF1

Plasmid

#65382

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF1 (BRPF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GFP-BRD1

Plasmid

#65375

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD1 (BRD1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pThy1-Autobow

Plasmid

#45187

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Autobow

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt4-V5

Plasmid

#43812

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHMGWA-Pa14Csy4

Plasmid

#41091

Depositor

Jennifer Doudna

Publication

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Expression

Bacterial

Mutation

Wild type

Promoter

T7

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pENTR221 LRRK2 wt

Plasmid

#39529

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Gateway compatible cloning vector

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only