We narrowed to 5,817 results for: pCas

1. mCherry-pCAG-mock

   Plasmid

   #228504

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-protein-mock

   Plasmid

   #228505

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCASCADE-LCAmp-trxB

   Plasmid

   #202465

   Purpose

   Expresses a guide RNA to silence trxB after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   trxB guide RNA
   

   Expression

   Bacterial

   Promoter

   ugpB

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-BiPOLES-mRuby3

   Plasmid

   #227780

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

   Depositor

   Hillel Adesnik

   Insert

   BiPOLES-mRuby3
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NG-ABE8.17-m+V106W

   Plasmid

   #198534

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-AGG-SpCas9

   Plasmid

   #214603

   Purpose

   encode SpCas9; in vitro transcribed modified RNA vector

   Depositor

   Yuxuan Guo

   Article

   Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

   Insert

   SpCas9
   

   Use

   CRISPR and Unspecified

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)

   Plasmid

   #173067

   Purpose

   Expresses NI-PE2

   Depositor

   Richard Sherwood

   Article

   Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

   Insert

   Igf1-NFATC2IP
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_SRGAP2A_SH3(W765A)_GFP

   Plasmid

   #222616

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_SRGAP2A_SH3(W765A)_HA

    Plasmid

    #222614

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSAVED-CHAT_PCaspase-N-term

    Plasmid

    #214044

    Purpose

    Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    aa 1-153

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSAVED-CHAT_PCaspase-C-term

    Plasmid

    #214045

    Purpose

    Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    aa 154-666

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSAVED-CHAT_PCaspase-N-C-term

    Plasmid

    #214046

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    aa 1-153, 154-666

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSAVED-CHAT-sm_PCaspase

    Plasmid

    #214048

    Purpose

    Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-EGFP-C-IFT46

    Plasmid

    #218724

    Purpose

    Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT46 (IFT46 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-spCas9-pZeo

    Plasmid

    #214121

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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