We narrowed to 1,479 results for: pbluescript

1. 564aa ELL2 bluescript

   Plasmid

   #127267

   Purpose

   For in vitro translation of human ELL2 lacking COOH region

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Addgene Plasmid 127256--SphI site filled in, then…

   Promoter

   T7

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

   Plasmid

   #122450

   Purpose

   Expression of inositol 1,4,5-trisphosphate 3-kinase A

   Depositor

   Christophe Erneux

   Article

   Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

   Insert

   inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
   

   Use

   Tags

   beta-galactosidase

   Expression

   Bacterial

   Mutation

   coding sequence from V66 till stop codon

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_miniENVLPE

   Plasmid

   #232431

   Purpose

   Expression plasmid to produce miniENVLPE particles

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   miniENVLPE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CTCF-mAC donor (Hygro)

   Plasmid

   #140646

   Purpose

   CTCF tagging with mAID-clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mAID-BRD4 donor

   Plasmid

   #140650

   Purpose

   BRD4 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-eTLR

   Plasmid

   #214737

   Purpose

   encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   AAVS1-eTLR (PPP1R12C CMV, Anguilla japonica, influenza, Bovine, Chicken, Rabbit, Synthetic, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CTCF-mAC donor (Neo)

   Plasmid

   #140645

   Purpose

   CTCF tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-I-Sce1-eno2:Tau-IRES-egfp

    Plasmid

    #112205

    Purpose

    Used to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoter

    Depositor

    Edward Burton

    Article

    Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

    Insert

    MAPT (MAPT Human)
    

    Use

    Zebrafish expression

    Tags

    eno2 exon 2 translational fusion, ires-egfp at c …

    Expression

    Mutation

    Promoter

    eno2 from zebrafish

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-f1(+)-SEC61B-mEGFP

    Plasmid

    #115910

    Purpose

    donor to insert EGFP at the SEC61B locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SEC61B-mEGFP (SEC61B Human)
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB71

    Plasmid

    #73085

    Purpose

    Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. iMb-Control-Mosaic (IR98.10)

    Plasmid

    #99748

    Purpose

    Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    MbYFP, MbTomato, MbKate2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36881

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMB37

    Plasmid

    #73084

    Purpose

    Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB73

    Plasmid

    #73086

    Purpose

    Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-f1(+)-TOMM20-mEGFP

    Plasmid

    #115911

    Purpose

    donor to insert EGFP at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-mEGFP (TOMM20 Human)
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid

    #105073

    Purpose

    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette

    Depositor

    Peter Mombaerts

    Article

    Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

    Insert

    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Drd1a coding sequence followed by IRES-tauGFP ACNF

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

    Plasmid

    #105196

    Purpose

    targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

    Depositor

    Peter Mombaerts

    Article

    Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

    Insert

    Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pBS-f1(+)-SEC61B-dTomato

    Plasmid

    #115913

    Purpose

    donor to insert dTomato at the SEC61B locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SEC61B-dTomato (SEC61B Human)
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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