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pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Th3.14

Plasmid

#188068

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 4 FuncLib mutations, described in publ…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FAM20C:V5

Plasmid

#63584

Purpose

FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

Depositor

Kenneth Irvine

Article

Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

Insert

FAM20C (Fam20c Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL3

Plasmid

#67460

Purpose

Mammalian expression of hArl3 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL1-GFP

Plasmid

#67395

Purpose

Mammalian expression of hArl1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pR2Q358y

Plasmid

#101028

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2.0 Tag-RFP-T-UtrCH

Plasmid

#101279

Purpose

Life imaging pCS2.0 Tag-RFP-T_UtrCH

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

Utrophin (UTRN Human)

Tags

TagRFP-T

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCR2.1_HBB_N

Plasmid

#87847

Purpose

pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

Depositor

Marina Kleanthous

Article

Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

Insert

Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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