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pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-V5-n2StayGoldC4

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

myr-mTagBFP2-Flag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mCherry-3xHA (JDW 1321)

Plasmid

#224493

Purpose

Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mCherry-3xHA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME TAEL2.0-5xC120-minpro (JDW 1109)

Plasmid

#224507

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL2.0-5xC120

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TAEL (JDW 1324)

Plasmid

#224508

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP-Cre (JDW 1329)

Plasmid

#224515

Purpose

A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME iCre-I (JDW 1203)

Plasmid

#224520

Purpose

A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

iCre-I

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E EFS-rtTA/rtTA3 (JDW 1214)

Plasmid

#224533

Purpose

A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA

Use

Gateway cloning

Promoter

EFS

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

Plasmid

#224534

Purpose

A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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