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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon optimized C-terminal 230 amino acids

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45b IRES GFP

Plasmid

#17881

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45b (Dpf1 Mouse)

Use

Tags

FLAG and IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-mBAF45c IRES GFP

Plasmid

#17882

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45c (Dpf3 Mouse)

Use

Tags

FLAG and IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
pIRES Centrin1 GFP Pur

Plasmid

#64341

Purpose

Bicistronic expression of Centrin-GFP fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1 (CETN1 Human)

Use

Tags

GFP and IRES-Puro

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-UBE3BΔIQ-HA-IRES-Puro

Plasmid

#107350

Purpose

Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BdelIQ (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-Blast SHMT2res

Plasmid

#106301

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Silent mutations destroy sgRNA targeting sites

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-A3A-seBE4max-IRES

Plasmid

#174698

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

A3A-seBE4max-IRES (APOBEC3A Human)

Use

Tags

myc tag

Expression

Mammalian

Mutation

A3A-M13I, UGI-E11K

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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