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Showing: 2161 - 2190 of 10866 results

  1. L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEV

    Plasmid
    #244188
    Purpose
    Multigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mNeonGreen; VEGFR2 NTEVp chain with NTEVp 75S mutant; VEGFR1 CTEVp chain with ZF6 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244188-plasmid-map-sequence-id-489497

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  2. L4426 IL-10Ra CTEVp chain with BATF in TUPV3

    Plasmid
    #244195
    Purpose
    Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hBATF
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and human BATF (BATF Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244195-plasmid-map-sequence-id-489494

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  3. L4427 IL-10Ra CTEVp chain with cJun in TUPV3

    Plasmid
    #244194
    Purpose
    Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hcJun
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and human cJun (JUN Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    244194-plasmid-map-sequence-id-489409

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  4. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244177
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    TNFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244177-plasmid-map-sequence-id-487904

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  5. pSCBE3-HF1-Hypa

    Plasmid
    #218158
    Purpose
    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218158-plasmid-map-sequence-id-433272

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  6. psiCHECK-VIM270-pSM

    Plasmid
    #215871
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215871-plasmid-map-sequence-id-431666

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  7. psiCHECK-VIM270-pCM

    Plasmid
    #215869
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215869-plasmid-map-sequence-id-431661

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  8. TFORF0362

    Plasmid
    #141589
    Purpose
    Lentiviral vector for overexpressing the TCEB2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCEB2 (ELOB Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141589-plasmid-map-sequence-id-409098

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  22. pDONR223-ZNF645

    Plasmid
    #88318
    Purpose
    Donor Vector containing ZNF645 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ZNF645 (ZNF645 Human)
    Use
    Gateway donor vector
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid
    #166814
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E1322R
    Promoter
    CMV
    Available Since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166814-plasmid-map-sequence-id-331962

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  24. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166811-plasmid-map-sequence-id-328312

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  25. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid
    #166812
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    H1276G
    Promoter
    CMV
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166812-plasmid-map-sequence-id-328391

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  26. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid
    #166813
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K1277E
    Promoter
    CMV
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166813-plasmid-map-sequence-id-328384

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Showing: 2161 - 2190 of 10866 results