We narrowed to 10,458 results for: T7
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Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
UseTagsExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX0393
Plasmid#205984PurposeGa0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacerDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1950
Plasmid#205983PurposeGa0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DRDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-Pom121-mScarlet
Plasmid#171491PurposeT7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNADepositorInsertPom121 (Pom121 Mouse)
UsePgehmeTagsmScarletExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mClover3
Plasmid#171489PurposeT7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNADepositorInsertH2B (H2BC11 Human)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(DAP10)
Plasmid#177904PurposeBacterial expression of his10-tagged human DAP10 Intracellular DomainDepositorInsertDAP10 (aa 70-92 only) (HCST Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelA12
Plasmid#176493PurposeExpresses the N-terminal His6-tagged SARS-CoV helicase in E coli.DepositorInsertSARS-CoV NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationV570IPromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_RBPMS2_FL
Plasmid#59394PurposePlasmid for bacterial overexpression of RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTags6xHis, FLAG, HA, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-ASD2HFD-GFP
Plasmid#237430PurposeExpresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T with ancestral HFD (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationHFD (residues 413-503) swapped from mouse:rat anc…PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-RpHFD-GFP
Plasmid#237428PurposeExpresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T with Rhabdomys pumilio HFD (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationHFD (residues 413-503) swapped from Rhabdomys pum…PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-McHFD-GFP
Plasmid#237437PurposeExpresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T with Mus caroli HFD (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationHFD (residues 413-503) swapped from Mus caroliPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-RnHFD-GFP
Plasmid#237429PurposeExpresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T with Rattus norvegicus HFD (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationHFD (residues 413-503) swapped from Rattus norveg…PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-MsHFD-GFP
Plasmid#237427PurposeExpresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationHFD (residues 413-503) swapped from Mus spretusPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
ATX3-JD
Plasmid#184247PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)DepositorInsertAtaxin-3 Josephin domain (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon V183XPromoterT7 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only