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pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-M2

Plasmid

#135583

Purpose

High eukaryotic protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-EGFP

Plasmid

#247324

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez, Clotilde Thery

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

CD63 (CD63 Human)

Tags

SBP-EGFP (inserted in the small luminal loop of C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDBB31

Plasmid

#248962

Purpose

DD-GAVPO (degron-regulated GAVPO) expression under control of the EF1alpha promoter in a PiggyBac integration plasmid

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Insert

DD-GAVPO

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-TeNT

Plasmid

#240990

Purpose

Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-TeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63YA-SBP-EGFP

Plasmid

#222327

Purpose

Synchronize the trafficking of CD63YA from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63-Y235A fused to SBP-EGFP (CD63 Human)

Expression

Mammalian

Mutation

Tyr235 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC036 (∆pHG1)

Plasmid

#226206

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for the pHG1 megaplasmid replication region

Use

Synthetic Biology

Mutation

∆helD ∆repA ∆repB ∆parAB

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDest-Sumo

Plasmid

#106980

Purpose

LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

Depositor

Han Choe

Article

Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

Type

Empty backbone

Tags

His8-Sumo

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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