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pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

Plasmid

#112683

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-iCre and Nuclear localization signal

Promoter

CMV-IE

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

Plasmid

#112719

Purpose

An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

AAV

Mutation

D10A

Promoter

Mecp2

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-BPTF-sh5

Plasmid

#83045

Purpose

MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #5 to BPTF (BPTF, Bptf Mouse, Human)

Use

Retroviral

Available Since

Oct. 24, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-MCU-mCerulean

Plasmid

#185809

Purpose

Human mitochondrial calcium uniporter with C-terminus mCerulean tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Promoter

CMV-IE

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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A3Bi-ctd E255A -Cas9n-UGI-NLS

Plasmid

#109429

Purpose

Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)

Use

CRISPR

Expression

Mammalian

Mutation

Insertion of an L1 intron into the coding sequenc…

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-BPTF-sh3

Plasmid

#73667

Purpose

pSIREN-RetroQ vector containing shRNA sequence to BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #3 to BPTF (BPTF, Bptf Mouse, Human)

Use

Retroviral

Available Since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hMer

Plasmid

#225996

Purpose

Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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