We narrowed to 2,368 results for: pcas9

1. pICSL1P1_AtU626Ter26

   Plasmid

   #191769

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWRS_1001

   Plasmid

   #192205

   Purpose

   lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

   Depositor

   William Sellers

   Article

   Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ABE-RA3.0

   Plasmid

   #208301

   Purpose

   Expresses ABE-RA3.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBT128c

   Plasmid

   #138512

   Purpose

   P2

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   inteinC-dSpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p206_LTJ_sgRNACD45.2_R3

   Plasmid

   #82674

   Purpose

   sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD45.2 region 3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p15A_SpyCas9_CmR

   Plasmid

   #119162

   Purpose

   Expresses S. pyogenes Cas9

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   Cas9 from S. pyogenes (NEWENTRY )
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ABE-RA2.0

   Plasmid

   #208299

   Purpose

   Expresses ABE-RA2.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA2.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ABE-RA3.1

   Plasmid

   #208302

   Purpose

   Expresses ABE-RA3.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.1-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ePE6d

    Plasmid

    #229029

    Purpose

    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

    Depositor

    Li Juan

    Article

    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px458_Conc2

    Plasmid

    #134449

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px458_Conc3

    Plasmid

    #134450

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p184_LTJ_sgRNACD90.2

    Plasmid

    #82670

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px459V2.0_Conc3

    Plasmid

    #134458

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.140

    Plasmid

    #184995

    Purpose

    Express Cas9 in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TetOn-3g

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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