Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,008 results for: SHA

Showing: 2191 - 2220 of 12008 results

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pH2B-miRFP704ano

Plasmid

#206196

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

H2B-miRFP704nano

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP704nano

Plasmid

#206195

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

LAMP1-miRFP704nano

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-mNeonC

Plasmid

#178461

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeonC

Plasmid

#178463

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus of the dimer

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Gal4-degFusionRed-p65

Plasmid

#199464

Purpose

Expression of the synthetic photodegradable transcription factor Gal4-degFusionRed-p65 in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-degFusionRed-p65

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-Pep-H

Plasmid

#185788

Purpose

Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-Pep4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-charge+

Plasmid

#160531

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336K, L340K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock

Plasmid

#160530

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-N342W

Plasmid

#160529

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-L340W

Plasmid

#160528

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-N338W

Plasmid

#160527

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-Y336W

Plasmid

#160526

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock-no charge

Plasmid

#160525

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, E337A, N338A, K341A, N342A

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-charge+

Plasmid

#160524

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB1lock

Plasmid

#160523

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-charge+

Plasmid

#160522

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-N342C

Plasmid

#160521

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-N342C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, N342C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-prepore-L340C

Plasmid

#160520

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-L340C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, L340C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-no charge

Plasmid

#160514

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

E337A, N338A, K341A, N342A

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-charge+

Plasmid

#160513

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18s-Ix_NPYLR1b

Plasmid

#138748

Purpose

Expresses Ixodes scapularis NPY-like receptor 1b (NPYLR1b) in a mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

Ixodes scapularis NPYLR1b (LOC8025400 Ixodes scapularis)

Expression

Mammalian

Promoter

SRa

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18s-Ix_NPYLR1a

Plasmid

#138716

Purpose

Expresses Ixodes scapularis NPY-like receptor 1a (NPYLR1a) in a mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR1a (LOC8025399 Ixodes scapularis)

Expression

Mammalian

Promoter

SRa

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2191 - 2220 of 12008 results

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