We narrowed to 4,300 results for: Pet2

1. Human Parkin (1-465)

   Plasmid

   #110758

   Purpose

   Bacterial expression of human Parkin

   Depositor

   David Komander

   Article

   Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

   Insert

   PRKN (PRKN Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpyTag-β-Lactamase-SpyCatcher

   Plasmid

   #52656

   Purpose

   Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

   Depositor

   Mark Howarth

   Article

   Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

   Insert

   TEM-1 β-Lactamase
   

   Use

   Tags

   SpyCatcher and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHis-hTG2

   Plasmid

   #100719

   Purpose

   Expression of human transglutaminase 2 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   silent mutation Arg580 (AGG--CGT) to enhance expr…

   Promoter

   T7

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-6xHis-(pos36)GFP

   Plasmid

   #62937

   Purpose

   Expression of 6xHis-(+36)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (pos36)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HTATIP (2OU2)

   Plasmid

   #33338

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HTATIP (KAT5 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. SENP2M497A

   Plasmid

   #61088

   Purpose

   cleave the SUMO off when use PETSUMO vector for express fusion protein

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   SENP2M497A catalytic domain (SENP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M497A; Catalytic domain is residues 364-589

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Ecoli PancACe

   Plasmid

   #215712

   Purpose

   Expresses acetyl-CoA biosensor in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEDA5_GFPmut3_Y66H

   Plasmid

   #80085

   Purpose

   Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

   Depositor

   Timothy Whitehead

   Article

   Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

   Insert

   GFPmut3_Y66H
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   changed Tyrosine 66 to Histidine

   Promoter

   proB

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B23(9-122)

    Plasmid

    #23142

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

    Insert

    B23 core (NPM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-8

    Promoter

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Pediculus humanus corporis PINK1 (115 - 575)

    Plasmid

    #110750

    Purpose

    Bacterial expression of PhPINK1 (biochemistry)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CTB

    Plasmid

    #141166

    Purpose

    Expresses recombinant Cholera Toxin B Subunit

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    CTB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No secretion signal

    Promoter

    T7

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCC25

    Plasmid

    #177319

    Purpose

    Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

    Depositor

    Rafal Tomecki

    Article

    Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

    Insert

    TtCutA 240-603
    

    Use

    Tags

    6xHis-SUMOTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SETDB1

    Plasmid

    #25240

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETDB1, SETDB1_16 (SETDB1 Human)
    

    Use

    Tags

    6xHis-TEV and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

21. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Promoter

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Human RNF144B (27-236)

    Plasmid

    #110753

    Purpose

    Bacterial Expression of RNF144B RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144B (RNF144B Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET sfGFP(150TAG)

    Plasmid

    #133455

    Purpose

    expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

    Depositor

    Yu-Hsuan Tsai

    Article

    Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

    Insert

    sfGFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDL17_Cas9-His Δcys M1C

    Plasmid

    #206251

    Purpose

    Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AMPKA2

    Plasmid

    #36194

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AMPKA2 (PRKAA2 Human)
    

    Use

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains KINASE DOMAIN only

    Promoter

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

27. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Use

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Use

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Fast-GCaMP-EF20

    Plasmid

    #52645

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF20
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D362N/D366N mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET-NEMO

    Plasmid

    #11966

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

31. Caspase-6

    Plasmid

    #100102

    Purpose

    Bacterial expression of Caspase-6

    Depositor

    Jim Wells

    Article

    Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

    Insert

    Caspase-6 (CASP6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJBL725

    Plasmid

    #140395

    Purpose

    Expresses SmtB protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-SmtB-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pJBL717

    Plasmid

    #140387

    Purpose

    Expresses MobR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MobR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-PLS-KP6_rpoA-U

    Plasmid

    #190967

    Purpose

    Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Human USP30 (64-502, construct 13, MG-26-82)

    Plasmid

    #110746

    Purpose

    Bacterial expression of human USP30 (construct 13)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350S, I353E + insertion deletion: 64-178;…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Use

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

42. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

44. HOXC13(human)-pET

    Plasmid

    #8679

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

45. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CXXC1

    Plasmid

    #32045

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC1 (CXXC1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    CXXC domain (amino acids residues 161-222)

    Promoter

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

47. DNMT3B (3QKJ)

    Plasmid

    #32044

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DNMT3B (DNMT3B Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    PWWP domain only (amino acids 293-442)

    Promoter

    Available Since

    Feb. 22, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

48. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

49. pJK357

    Plasmid

    #71568

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge