Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,964 results for: BASE;

Clear keywords
Showing: 1461 - 1480 of 12964 results

  3. SpCas9-NG-ABE8.17-m+V106W

    Plasmid
    #198534
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8.17-m+V106W
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198534-plasmid-map-sequence-id-388875

    Enlarge

  5. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

    Enlarge

  7. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

    Enlarge

  13. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

    Enlarge

  15. GANLS

    Plasmid
    #50852
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50843-plasmid-map-sequence-id-76292

    Enlarge

  18. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

    Enlarge

Showing: 1461 - 1480 of 12964 results