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Plasmid#203736PurposeOverexpression of ALKBH5-WTDepositorInsertALKBH5 (ALKBH5 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
RN3P-KDM5B-FLAG
Plasmid#86398PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expressionDepositorInsertKDM5B (Kdm5b Mouse)
UseTags3x-FLAGExpressionMammalianMutationPromoterLacAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-PABPC1
Plasmid#232944PurposeExpresses PABPC1 proteinDepositorInsertPABPC1 (PABPC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGG L2 TaStb15
Plasmid#226630PurposeWheat transformation vector used to express wheat Septoria tritici blotch (Stb) resistance gene 15DepositorInsertTriticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene
UseSynthetic BiologyTagsExpressionPlantMutationPromoterNative TaStb15Available sinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3-NG-HF1
Plasmid#218160PurposeThis plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsscbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutations in the portion of SpCas9 : D10A / N497A…PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3-NG-Hypa
Plasmid#218161PurposeThis plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsscbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutations in the portion of SpCas9 : D10A / N692A…PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)
Plasmid#232947PurposeExpresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdownDepositorInsertYTHDF2-D-MUT (YTHDF2 Human)
UseLentiviralTagsExpressionMutation5 site mutation (Y418A+D422A+W432A+W486A+W491A), …PromoterAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)
Plasmid#232945PurposeExpresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdownDepositorInsertYTHDF2 (YTHDF2 Human)
UseLentiviralTagsExpressionMutationsynonymous mutationPromoterAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-YTHDF2-m(6)A-MUT (nonsense mutation of sgYTHDF2-1)
Plasmid#232946PurposeExpresses the m6A-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdownDepositorInsertYTHDF2-m(6)A-MUT (YTHDF2 Human)
UseLentiviralTagsExpressionMutation2 site mutation (W432A+W486A), synonymous mutationPromoterAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-Hypa
Plasmid#218165PurposeThis plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in StreptomycesDepositorInsertsescbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDH-Mut-ALKBH5-3xFLAG
Plasmid#203737PurposeOverexpression of ALKBH5-MutDepositorInsertALKBH5 (ALKBH5 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationEnzyme Inactive Mutation (H226A)PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-YTHDF2-m6A-MUT
Plasmid#238976PurposeExpresses the m6A-binding-deficient mutant of YTHDF2DepositorInsertYTHDF2-m6A-MUT (YTHDF2 Human)
UseLentiviralTagsExpressionMammalianMutation2 site mutation (W432A+W486A)PromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorUseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorUseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1
Plasmid#218164PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_WT
Plasmid#108922PurposeThe core promoter region of FTO was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_Mut
Plasmid#108923PurposeThe core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-REPORT-MYC CDS_WT
Plasmid#108924PurposeMYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vectorDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3_Flag-YTHDF2-RNA-MUT
Plasmid#238978PurposeExpresses the RNA-binding-deficient mutant of YTHDF2DepositorInsertYTHDF2-RNA-MUT (YTHDF2 Human)
UseLentiviralTagsExpressionMammalianMutationRNA binding mutation (K416A+R527A)PromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only