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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10V5

Plasmid

#181938

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xV5

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10Myc

Plasmid

#181939

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xMyc

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Ty:mScarletI:3Ty

Plasmid

#201090

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-His:mNG:His

Plasmid

#201096

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Use

Tags

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Promoter

Available since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3Myc

Plasmid

#221046

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xMyc epitopes

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG

Plasmid

#179817

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

HRE-mCMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Myc:mNG:3Myc

Plasmid

#179803

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-CLIPtag

Plasmid

#179787

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

CLIP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

p23

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(V97T)

Plasmid

#197944

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(G41)

Plasmid

#197942

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

Plasmid

#197943

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Cdk5

Plasmid

#24894

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

CDK5 (CDK5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2010

Availability

Academic Institutions and Nonprofits only