We narrowed to 15,796 results for: form

1. pcDNA3.1-39S Light chain

   Plasmid

   #216297

   Purpose

   Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   39S Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MF3958 Light chain

   Plasmid

   #216304

   Purpose

   Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MF3958 Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

   Plasmid

   #201187

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 2 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

   Plasmid

   #201188

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 3 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. V5-K202R-short-UBASH3A

   Plasmid

   #192084

   Purpose

   Mammalian expression of human K202R short-form UBASH3A with V5 tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   K202R

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-W279A-short-UBASH3A-Ntag

   Plasmid

   #192096

   Purpose

   Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W279A

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-K202R-short-UBASH3A-Ntag

   Plasmid

   #192094

   Purpose

   Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K202R

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-WT-short-UBASH3A-Ctag

   Plasmid

   #192099

   Purpose

   Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-WT-short-UBASH3A-Ntag

   Plasmid

   #192100

   Purpose

   Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-preSufI(IAC)

    Plasmid

    #168516

    Purpose

    Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    pre-SufI(IAC)
    

    Use

    Tags

    6xHisC tag and HSV tag

    Expression

    Bacterial

    Mutation

    C17I and C295A

    Promoter

    T7

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. NK85

    Plasmid

    #176611

    Purpose

    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    FCERG
    

    Use

    Lentiviral

    Tags

    EGFP and HA, SNAP-tag

    Expression

    Mammalian

    Mutation

    C25A

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. C413-E40: attB4-EFSp>-attB1r

    Plasmid

    #162928

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    short form of human EF1 alpha promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK274

    Plasmid

    #71564

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 3)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1 promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP64T3 BMP2 pro dnmVg1 (DM#182)

    Plasmid

    #15074

    Depositor

    Douglas Melton

    Article

    Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

    Insert

    BMP2-Vg1 dom neg
    

    Use

    Sp6 expression for use in xenopus

    Tags

    Expression

    Mutation

    dominant negative version of BMP2-Vg1 chimera. Th…

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-TPNOX

    Plasmid

    #87853

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    TPNOX
    

    Use

    Tags

    C-terminal FLAG tag with a linker

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. modRNAc0-p53DD

    Plasmid

    #176902

    Purpose

    IVT template plasmid for making p53DD modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    p53DD
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-HpaBC

    Plasmid

    #71260

    Purpose

    Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

    Inserts

    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. RAB14 S25N

    Plasmid

    #61495

    Purpose

    to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S25N, inactive form

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only