Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,246 results for: golden gate

Clear keywords
Showing: 1461 - 1480 of 5246 results

  5. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

    Enlarge

  6. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

    Enlarge

  12. HSA_3b*_AP

    Plasmid
    #238899
    Purpose
    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Publication
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    Recombinant Human Serum Albumin (HSA) 3b*
    Use
    Synthetic Biology
    Available since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    238899-plasmid-map-sequence-id-482774

    Enlarge

  15. pKEK3230

    Plasmid
    #234102
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Publication
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    234102-plasmid-map-sequence-id-471768

    Enlarge

  17. pKEK3235

    Plasmid
    #234107
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Publication
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234107-plasmid-map-sequence-id-467990

    Enlarge

  18. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Publication
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

    Enlarge

Showing: 1461 - 1480 of 5246 results