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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 148,763 results for: ins;

Showing: 1461 - 1480 of 148763 results

  1. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  2. pCCG_nit9E

    Plasmid
    #228419
    Purpose
    Expression of NIT-9E domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of the N terminal G domain and linkage…
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228419-plasmid-map-sequence-id-460292

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  3. pCCG_nit9G

    Plasmid
    #228420
    Purpose
    Expression of NIT-9G domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of the C terminal E domain and linkage…
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228420-plasmid-map-sequence-id-460291

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  4. pCCG_nit9_A184-G203

    Plasmid
    #228421
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of A184-G203
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228421-plasmid-map-sequence-id-460290

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  5. pCCG_nit9_G204-G223

    Plasmid
    #228422
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of G204-G223
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228422-plasmid-map-sequence-id-460288

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  6. pCCG_nit9_E224-G243

    Plasmid
    #228423
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of E224-G243
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228423-plasmid-map-sequence-id-460277

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  7. pCCG_nit9_K264-P282

    Plasmid
    #228425
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of K264-P282
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228425-plasmid-map-sequence-id-460271

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  8. pCCG_nit9G_linker

    Plasmid
    #228413
    Purpose
    Expression of NIT-9G with linkage region at the C terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of the C terminal E domain
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228413-plasmid-map-sequence-id-459453

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  9. pCCG_linker_nit9E

    Plasmid
    #228412
    Purpose
    Expression of NIT-9E with linkage region at the N terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    truncation of the N terminal G domain
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228412-plasmid-map-sequence-id-459452

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  10. pCCG_nit9_without_linker

    Plasmid
    #228411
    Purpose
    Expression of NIT-9 without linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Mutation
    deletion of the linkage region
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228411-plasmid-map-sequence-id-459451

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  15. pTipi2.1-AZU_SP

    Plasmid
    #229144
    Purpose
    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.
    Depositor
    Institute for Protein Innovation (IPI)
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    229144-plasmid-map-sequence-id-457154

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Showing: 1461 - 1480 of 148763 results