We narrowed to 2,414 results for: organization Addgene

1. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   longCaMV35S

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZJOM18

   Plasmid

   #133631

   Purpose

   Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-NAB2-GFP (NAB2 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUF-AsCpf1-pre-gRNA

   Plasmid

   #137849

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1 and pre-gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUF-LbCpf1-pre-gRNA

   Plasmid

   #137848

   Purpose

   LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   LbCpf1-NLS-FLAG and gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NPC1-CFP P692S

   Plasmid

   #58457

   Purpose

   expression of NPC1-CFP sterol sensing domain mutant

   Depositor

   Matthew Scott

   Article

   Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

   Insert

   NPC1 (Npc1 Mouse)
   

   Use

   Tags

   ECFP and His6

   Expression

   Mammalian

   Mutation

   P692S sterol sensing domain mutant

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV 4xSTAR-mTFP-NLS-blast

   Plasmid

   #136257

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTFP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKV016

   Plasmid

   #64767

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM41-EcPPXc

   Plasmid

   #38329

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Use

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. KG#371

    Plasmid

    #110882

    Purpose

    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

    Inserts

    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Use

    Tags

    intron-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Keratin 8 S73A

    Plasmid

    #18056

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S73A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNS1

    Plasmid

    #131769

    Purpose

    PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    FUSN_Fusion Red_Cry2WT
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PADH1

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Keratin 8 S73D

    Plasmid

    #18054

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S73D

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pMH-HT_CtCHAD

    Plasmid

    #127785

    Purpose

    Expresses CtCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Keratin 8 S431A

    Plasmid

    #18058

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S431A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pFA-sbtA

    Plasmid

    #133011

    Purpose

    pFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    SbtA
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human K8

    Plasmid

    #14742

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only