We narrowed to 1,518 results for: pet-28a

1. CFP-FRBx5-His

   Plasmid

   #103784

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   CFP-FRBx5-His
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFP-FKBPx5-His

   Plasmid

   #103785

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-FKBPx5-His
   

   Use

   Tags

   EYFP, His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-Bsa4-ICL1

   Plasmid

   #105822

   Purpose

   Expression of Mycobacterium tuberculosis icl1 recombinant protein

   Depositor

   Ivanhoe Leung

   Article

   Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

   Insert

   icl1
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZ004 (LseC2c2 locus)

   Plasmid

   #70170

   Purpose

   Expresses LseC2c2 locus with one spacer in array

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LseC2c2 locus with one spacer in array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

   Plasmid

   #110747

   Purpose

   Bacterial expression of human USP30 (construct 13i)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   C77A, F348D, M350S, I353E + insertion deletion: 6…

   Promoter

   T7

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. hVAMP3(1-77) S44A

   Plasmid

   #206029

   Purpose

   Expresses the SNARE motif of human VAMP3 mutant S44A in bacteria

   Depositor

   Geert van den Bogaart

   Article

   Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

   Insert

   Vesicle-associated membrane protein 3 (VAMP3 Human)
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   changes serine 44 to alanine

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. hVAMP3(1-77) S44E

   Plasmid

   #206030

   Purpose

   Expresses the SNARE motif of human VAMP3 mutant S44E in bacteria

   Depositor

   Geert van den Bogaart

   Article

   Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

   Insert

   Vesicle-associated membrane protein 3 (VAMP3 Human)
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   changes serine 44 to glutamic acid

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

   Plasmid

   #243822

   Purpose

   Munc13-1 protein purification

   Depositor

   Jose Rizo-Rey

   Article

   Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

   Insert

   Munc13-1 (Unc13a Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   delta1408-1452 delta1533-1551 R1598E F1658E

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLyGo-Ec-4

    Plasmid

    #163130

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK287

    Plasmid

    #71120

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCeSR beta deltaN

    Plasmid

    #13728

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (R186.3 Nematode)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    expresses residues 38-240 of C. elegans SRβ with …

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScSR beta deltaN Flag

    Plasmid

    #13729

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    expresses residues 31-244 of S. cerevisiae SRβ wi…

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. tUI

    Plasmid

    #122661

    Purpose

    Express tUI protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI (UBQLN1, USP5 Human)
    

    Use

    Tags

    His-tag

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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