We narrowed to 9,511 results for: BLI;
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Plasmid#211460PurposeExpress mitochondrial localized ApaLI with E225A and K227A mutationsDepositorInsertApaLI-E225A-K227A
TagsHAExpressionMammalianMutationE225A, K227APromoterCMVAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
ExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_69.11
Plasmid#194214PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK3 Ankyrin Repeats pET151
Plasmid#164056PurposeExpresses mouse KANK3 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4 Ankyrin Repeats pET151
Plasmid#164057PurposeExpresses mouse KANK4 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox
Plasmid#182360PurposeShuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2DepositorInsertMyr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
UseCre/Lox, Mouse Targeting, and Synthetic Biology ;…ExpressionMammalianAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812_2PA
Plasmid#105676PurposeExpresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 bindingDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only