We narrowed to 875 results for: seph

1. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

   Plasmid

   #133800

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized Eriv-AcrIIA1
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV10/3xflag-Clock L113E

   Plasmid

   #47339

   Purpose

   Mutation used for co-IPs

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   L113E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCMV10/3xflag-Clock F122D

   Plasmid

   #47340

   Purpose

   Mutation used for co-IPs

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   F122D

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3-HA/BmalF423R

   Plasmid

   #47348

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   F423R

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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