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Plasmid#176229PurposeA template plasmid for amplification of the cI-hok-aacC4 cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, aacC4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPAdGO2-PGK-Neo
Plasmid#136899PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPAdGO2
UseLentiviralTags2xNLSExpressionMutationTGG>TAGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCRi
Plasmid#207524PurposePlasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
UsePseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
UseTagsExpressionBacterialMutationTtgR: R130H A144T A148VPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAID-EF1a linearizing in pX330
Plasmid#140610PurposeCrispr/Cas9 plasmid for linearization of pAID-EF1aDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pRRL-GFPAdGO-PGK-Neo
Plasmid#136898PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPAdGO
UseLentiviralTags2xNLSExpressionMutationTGG>TGAPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
UseTagsExpressionYeastMutationPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSe-Rp1
Plasmid#111644PurposeFor construction of spacer and repeat arrays for Sulfolobus islandicus REY154ADepositorTypeEmpty backboneUseArchaeal expression vectorTagsExpressionMutationPromoteraraSAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B4
Plasmid#31021DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B2
Plasmid#31019DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
dCas9-AsLOV2x3-NLSx3
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ959
Plasmid#192603PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterScTEF1Available sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only