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pRK5-HA-SZT2

Plasmid

#87035

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-RFP

Plasmid

#22907

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

human Synapsin I promoter driving DsRedExpress (SYN1 Human)

Use

AAV; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-NES-RFP

Plasmid

#221812

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NES-mCherry

Use

Lentiviral

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-fNPY-GFP

Plasmid

#22912

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu neuropeptide Y promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PmxaF

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2

Plasmid

#87034

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pEF6 VPS52-V5-6His

Plasmid

#175096

Purpose

V5 tagged VPS52 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

VPS52 (VPS52 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

N666D- please see depositor comment

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pTE105

Plasmid

#59400

Purpose

MethyloBrick expression vector, tuf promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptuf

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pTE101

Plasmid

#59396

Purpose

MethyloBrick cloning vector, no promoter; KmR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE103

Plasmid

#59398

Purpose

MethyloBrick expression vector, fumC promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PfumC

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-Nprl2

Plasmid

#87051

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

Nprl2 (NPRL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQE80l A-CC-A

Plasmid

#196478

Purpose

Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

A-CC-A

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTE104

Plasmid

#59399

Purpose

MethyloBrick expression vector, coxB promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PcoxB

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pOGG010

Plasmid

#113984

Purpose

Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

RK2 (IncP)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFIN-NRLL-tdTOM

Plasmid

#44355

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

NRLL-tdTOM

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mouse neural leucine zipper (pos 3127-5587)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-fCR-GFP

Plasmid

#22911

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu calretinin promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only