We narrowed to 7,351 results for: ski

1. pAAV-EFS-hfCas13d-HA-mCherry-pA

   Plasmid

   #233029

   Purpose

   To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   hfCas13d-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EFS-DjCas13d-P2A-mCherry-pA

   Plasmid

   #233027

   Purpose

   To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   DjCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EFS-CasRx-P2A-mCherry-pA

   Plasmid

   #233025

   Purpose

   To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EFS-hfCas13d-P2A-mCherry-pA

   Plasmid

   #233026

   Purpose

   To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   hfCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

   Plasmid

   #233032

   Purpose

   To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx and mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p204-U6-Switch-ON

   Plasmid

   #217883

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

   Plasmid

   #195865

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Yes: GTGGAATACATTACCAACGTGGTGTACGTG

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-intron-DIO-hfCas13d-pA

   Plasmid

   #195866

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Yes: GTGGAATACATTACCAACGTGGTGTACGTG

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NLS-tdPCP-GFP-VP16

    Plasmid

    #183935

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57-attB2-SA(1)-CRY2-mCherry

    Plasmid

    #160440

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57-attB2-SA(0)-CRY2-mCherry

    Plasmid

    #160439

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 BMPR1A

    Plasmid

    #67105

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR1A (BMPR1A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 EHD2

    Plasmid

    #67107

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EHD2 (EHD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 GTF2H1

    Plasmid

    #67108

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRevLCav2FeS

    Plasmid

    #60808

    Purpose

    to disrupt iron sulfur cluster in yeast Rev3

    Depositor

    Tahir Tahirov, Youri Pavlov

    Article

    Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Promoter

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBABEpuro-ARAF_D429A

    Plasmid

    #51080

    Purpose

    mammalian expression of ARAF D429A

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D429A

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX-Frb:Akt2-IRES-Hygromycin

    Plasmid

    #120713

    Purpose

    Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    AKT2 (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge