We narrowed to 7,531 results for: ski

1. pRevLCav2FeS

   Plasmid

   #60808

   Purpose

   to disrupt iron sulfur cluster in yeast Rev3

   Depositor

   Youri Pavlov, Tahir Tahirov

   Article

   Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

   Insert

   REV3 (REV3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   contains 647 C-terminal amino acids and 335 bp of…

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBABEpuro-ARAF_D429A

   Plasmid

   #51080

   Purpose

   mammalian expression of ARAF D429A

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D429A

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-Gluc-RMA-IRES-EGFP

   Plasmid

   #189629

   Purpose

   Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Promoter

   IRES and hSyn

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mfn2-YFP

   Plasmid

   #28010

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

   Insert

   mitofusin 2 (MFN2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   silent S508S

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

   Plasmid

   #233036

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

   Plasmid

   #192481

   Purpose

   To express CasRX and a gRNA

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   CasRx
   

   Use

   AAV

   Mutation

   No

   Promoter

   U6/EFS

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-intron-hfCas13d-pA

   Plasmid

   #195864

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Mutation

   Yes: GTGGAATACATTACCAACGTGGTGTACGTG

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

    Plasmid

    #222852

    Purpose

    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-GP-2-KDM4A double Tudor

    Plasmid

    #59699

    Purpose

    Contains histone modification interacting domain (KDM4A double Tudor)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    KDM4A double Tudor (KDM4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. A*24:02:01

    Plasmid

    #203295

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:02:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR-attL5-CRY2-mCh-attL2

    Plasmid

    #160438

    Purpose

    Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDsRed1-CMV-Rat Arc-DsRed1-pA

    Plasmid

    #233049

    Purpose

    To Express a Rat Arc-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

    Insert

    rat Arc-DsRed fusion (Arc Rat)
    

    Tags

    DsRed1

    Expression

    Mammalian

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-DCX-TurboID-V5

    Plasmid

    #190740

    Purpose

    Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    doublecortin (DCX Human)
    

    Tags

    TurboID and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. A*11:01:01:01

    Plasmid

    #203276

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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