We narrowed to 10,632 results for: t7

1. pET23a_mod_RBPMS2_FL

   Plasmid

   #59394

   Purpose

   Plasmid for bacterial overexpression of RBPMS2

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS2 (RBPMS2 Human)
   

   Tags

   6xHis, FLAG, HA, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-MmCENPT-ASD2HFD-GFP

   Plasmid

   #237430

   Purpose

   Expresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with ancestral HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from mouse:rat anc…

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-MmCENPT-RpHFD-GFP

   Plasmid

   #237428

   Purpose

   Expresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Rhabdomys pumilio HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from Rhabdomys pum…

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-MmCENPT-McHFD-GFP

   Plasmid

   #237437

   Purpose

   Expresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Mus caroli HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from Mus caroli

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-MmCENPT-RnHFD-GFP

   Plasmid

   #237429

   Purpose

   Expresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Rattus norvegicus HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from Rattus norveg…

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE-MmCENPT-MsHFD-GFP

   Plasmid

   #237427

   Purpose

   Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from Mus spretus

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATX3-JD

   Plasmid

   #184247

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain (ATXN3 Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon V183X

   Promoter

   T7 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATX3-D1

   Plasmid

   #184246

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon N263X

   Promoter

   T7 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-bNup98-DN-NLS-mClover3

   Plasmid

   #171495

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Nup98-DN (NUP98 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a Ec-iPGM-bio

    Plasmid

    #162575

    Purpose

    Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    E. coli iPGM bio-6His
    

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJV126

    Plasmid

    #124250

    Purpose

    In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJV127

    Plasmid

    #124251

    Purpose

    In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJV128

    Plasmid

    #124252

    Purpose

    In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJV129

    Plasmid

    #124253

    Purpose

    In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(cd96)

    Plasmid

    #177901

    Purpose

    Bacterial expression of his10-tagged human cd96 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD96 (aa 557-585 only) (CD96 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHyperTEV60

    Plasmid

    #228496

    Purpose

    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    TEV Protease
    

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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