We narrowed to 4,045 results for: golden gate

Showing: 3751 - 3800 of 4045 results

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DG SaCas9 GFP V3

Plasmid

#226961

Purpose

CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-109

Plasmid

#218200

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-858

Plasmid

#218008

Purpose

mVenus fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mVenus

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-853

Plasmid

#218011

Purpose

mCerulean fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-854

Plasmid

#218012

Purpose

mVenus fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mVenus

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-855

Plasmid

#218013

Purpose

mCherry fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCherry

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-856

Plasmid

#218014

Purpose

sfGFP fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

sfGFP

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-872

Plasmid

#218015

Purpose

NanoLuc luminescence reporter

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

NanoLuc

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-860

Plasmid

#218016

Purpose

mVenus fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mVenus

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-861

Plasmid

#218017

Purpose

mCerulean fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-871

Plasmid

#218019

Purpose

8xHis-1xHA-tag for protein detection and purification

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-1xHA-tag

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-1022

Plasmid

#218020

Purpose

8xHis-SP20-3xHA-tag for protein detection and purification

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-SP20-1xHA-tag

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-869

Plasmid

#218021

Purpose

ER retention signal (DGDL) for protein targeting to ER

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

ER retention signal (DGDL)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-101

Plasmid

#218199

Purpose

Multi-Stop for adding 3 stops for each frame

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Multi-Stop

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-099

Plasmid

#218201

Purpose

Strep-8xHis-tag for protein detection and purification

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-8xHis

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-100

Plasmid

#218202

Purpose

3xHA epitope tag for protein detection

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xHA

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-723

Plasmid

#218203

Purpose

1xHA-8xHis-tag for protein detection and purification

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

1xHA-8xHis

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-054

Plasmid

#218204

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-838

Plasmid

#217994

Purpose

pLEXSY_I-blecherry3_dom_lacZ Destination Vector

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

T7 promotor

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-840

Plasmid

#217996

Purpose

3xHA epitope tag for protein detection

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xHA

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-843

Plasmid

#217997

Purpose

smtHSP60 for protein targeting to mitochondrial matrix

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

smtHSP60

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-844

Plasmid

#217998

Purpose

Nucleoplasmin NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Nucleoplasmin NLS

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-1125

Plasmid

#217999

Purpose

Start-methionine

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Start-Met

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-846

Plasmid

#218001

Purpose

3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xFLAG-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-847

Plasmid

#218002

Purpose

Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Myc-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-851

Plasmid

#218006

Purpose

Spacer to bridge position B3

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Spacer

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS2

Plasmid

#184885

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS3

Plasmid

#184886

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS1

Plasmid

#184884

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-mini v2-HF24_F4

Plasmid

#195718

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#4 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F20

Plasmid

#195734

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#20 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F12

Plasmid

#195726

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#12 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F3

Plasmid

#195717

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#3 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F24

Plasmid

#195738

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#24 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F7

Plasmid

#195721

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#7 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F8

Plasmid

#195722

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#8 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F14

Plasmid

#195728

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#14 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F13

Plasmid

#195727

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#13 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F22

Plasmid

#195736

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#22 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F10

Plasmid

#195724

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#10 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F6

Plasmid

#195720

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#6 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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We narrowed to 4,045 results for: golden gate