We narrowed to 5,049 results for: golden gate

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pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG2942

Plasmid

#246346

Purpose

ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF37-2A-mCherry-BGH_pKG2943

Plasmid

#246347

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-mCherry from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiX_EF1a-VP16-ZF37-2A-TagBFP-WPRE_pKG3675

Plasmid

#246348

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiX_PGK-VP16-ZF37-2A-TagBFP-WPRE_pKG3958

Plasmid

#246349

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the hPGK promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787

Plasmid

#246327

Purpose

loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMF1-M

Plasmid

#242203

Purpose

OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

OsU6-2p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF2-M

Plasmid

#242204

Purpose

OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

OsU6-2p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF1-D

Plasmid

#242205

Purpose

AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU6-26p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFC544

Plasmid

#246441

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-rtTa

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-rtTa

Use

Synthetic Biology

Promoter

miniCMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV712

Plasmid

#246444

Purpose

The plasmid comprises an indicible transcriptional unit encoding EYFP

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

EYFP

Use

Synthetic Biology

Promoter

miniTK

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV742

Plasmid

#246447

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV714

Plasmid

#246445

Purpose

The plasmid comprises an indicible transcriptional unit encoding EYFP

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

EYFP

Use

Synthetic Biology

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV715

Plasmid

#246446

Purpose

The plasmid comprises an indicible transcriptional unit encoding EYFP

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

EYFP

Use

Synthetic Biology

Promoter

miniCMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFC546

Plasmid

#246443

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-YY1

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-YY1

Use

Synthetic Biology

Promoter

miniCMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFC543

Plasmid

#246440

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Promoter

miniCMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CBE-Cas12a-GFP

Plasmid

#246533

Purpose

Plant co-editing vector

Depositor

Nian Wang

Article

Huang et al Nat Plants. 2023 Oct;9(10):1591-1597. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFC545

Plasmid

#246442

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-Hsf1

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-Hsf1

Use

Synthetic Biology

Promoter

miniCMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA1540

Plasmid

#246061

Purpose

Bacterial expression of 2TMbeta protein under the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

2TMbeta

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

Plasmid

#242152

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

Plasmid

#242153

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5 S170 LOV

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNS1D-sgRNA9-SpR

Plasmid

#191092

Purpose

aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

Depositor

Anne Ruffing

Article

Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

tet

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAD183_gCAD_pET

Plasmid

#239119

Purpose

Gorilla ACOD1 in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

StrepTag-II and TEV site

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

Plasmid

#239727

Purpose

Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

Plasmid

#239734

Purpose

Plasmid expressing tagBFP with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

Plasmid

#239735

Purpose

Plasmid expressing tagBFP with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH

Plasmid

#239736

Purpose

Plasmid expressing tagBFP with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH

Plasmid

#239737

Purpose

Plasmid expressing tagBFP with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH

Plasmid

#239738

Purpose

Plasmid expressing tagBFP with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2023_pGEEC509_pShip-UbC-mRuby2-WPRE

Plasmid

#239715

Purpose

Plasmid expressing mRuby2 with the UbC promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH

Plasmid

#239716

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40

Plasmid

#239717

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE

Plasmid

#239718

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2075_pGEEC516_pShip-CAG-mRuby2-bGH

Plasmid

#239719

Purpose

Plasmid expressing mRuby2 with the CAG promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2076_pGEEC517_pShip-CAG-mRuby2-SV40

Plasmid

#239720

Purpose

Plasmid expressing mRuby2 with the CAG promoter and SV40 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2077_pGEEC518_pShip-CAG-mRuby2-WPRE

Plasmid

#239721

Purpose

Plasmid expressing mRuby2 with the CAG promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH

Plasmid

#239722

Purpose

Plasmid expressing a grazoprevir-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2215_pGEEC525_pShip-ZF43x6-C-mRuby2-bGH

Plasmid

#239723

Purpose

Plasmid expressing mRuby2 from a COMET promoter, Induced with pKG3666

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2410_pGEEC526_pShip-TRE3G-mRuby2-bGH

Plasmid

#239724

Purpose

Plasmid expressing mRuby2 from the TRE3G promoter, Induced with pKG3668

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2497_pGEEC529_pShip-CMV-mRuby2-bGH

Plasmid

#239725

Purpose

Plasmid expressing mRuby2 with the CMV promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 5,049 results for: golden gate