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pCri-4a-Strep

Plasmid

#61338

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag, Strep-tag, and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-1a-Strep

Plasmid

#61337

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MBP-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A(KaganE18)

Plasmid

#52009

Purpose

Expresses the human MAVS CDS containing the point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 1 to Ala

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M142A(KaganE19)

Plasmid

#52010

Purpose

Expresses the human MAVS CDS containing the point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A(KaganF62)

Plasmid

#52002

Purpose

Expresses the human MAVS CDS containing two point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A(KaganF59)

Plasmid

#52062

Purpose

Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP2 GFP

Plasmid

#51886

Purpose

expression of Xl-VSP2-GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00002

Plasmid

#50347

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pQC_HA-p115 TEV-Myc #77#407

Plasmid

#42127

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

inserted ENLYFQ b/w 886 and 887

Promoter

CMV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
27 pCR plcg 1 CDS

Plasmid

#13058

Depositor

Nathan Lawson

Article

Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

Insert

plcg1 (plcg1 Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper delta ITAM

Plasmid

#126671

Purpose

Expression of His10-Draper delta ITAM mutant for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic ITAM residues mutated to Phe

Expression

Bacterial

Mutation

Cytoplasmic ITAM residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pNGVL4a-hCRTmE6mE7mL2

Plasmid

#104153

Purpose

Expresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cells

Depositor

Richard Roden

Article

Jiang et al J Virol. 2017 Jul 12;91(15). pii: e00699

Insert

hCRTmE6mE7mL2 11-210 (CALR, E6, E7, L2 Human, MmuPV1)

Expression

Mammalian

Mutation

residues 11 to 200 of the late protein L2

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Tags

N-terminal mScarlet tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRSV-Rev

Plasmid

#12253

Purpose

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

Rev (rev )

Use

Lentiviral; Packaging

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Blast

Plasmid

#52962

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Has Service

Lentiviral Prep

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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