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Plasmid#113083PurposeEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pEY52
Plasmid#191055Purposeodr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-1 (odr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEY48
Plasmid#191052Purposeklp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertklp-6 (klp-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY47
Plasmid#191051Purposegcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-21 (gcy-21 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-RFP-RGG
Plasmid#124934PurposeExpression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEY15
Plasmid#191021Purposeflp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-1 (flp-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY12
Plasmid#191018PurposeFluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorInsertrab-3 (rab-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY46
Plasmid#191050Purposeglr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertglr-1 (glr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY38
Plasmid#191042Purposeunc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertunc-8 (unc-8 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY13
Plasmid#191019PurposeFluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorInsertarrd-4 (ZK938.2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY20
Plasmid#191026Purposegcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-18 (gcy-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWP040
Plasmid#198777PurposeDrives specific expression in the ASG neuronsDepositorInsertPY41C4A.6-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC12
Plasmid#198767PurposeDrives specific expression in the ADF neuronsDepositorInsertPsrh-142-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP017
Plasmid#198780PurposeDrives specific expression in the ASI neuronsDepositorInsertPgpa-4-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNF-5 pXOOM
Plasmid#48697PurposeExpressed SNF-5 in Xenopus oocytes or mammalian cellsDepositorInsertsnf-5 (snf-5 Nematode)
UseXenopus oocyte expressionTagsExpressionMammalianMutationK42E (when compared to NP_496735.1)PromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY22
Plasmid#191028Purposelim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertlim-4 (lim-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only