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TET-pLKO.1 PURO shId2 #1

Plasmid

#83088

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pSC5-toxic3

Plasmid

#188625

Purpose

pSC5GGv2 with HindII Gene Insert

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Insert

HindII

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

ACT1 intron insert

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDA2ScIfm

Plasmid

#193786

Purpose

Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLTATAA synthetic promoter carrying binding sites…

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

Plasmid

#199254

Purpose

Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KARA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A R1060A

Promoter

CAG promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLA2ScIfmTcIfm

Plasmid

#193792

Purpose

Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLLacO-1 and PLTetO-1

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R

Plasmid

#147431

Purpose

Insect Expression of DmNot1_1147-1717-K1277A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717-K1277A (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R

Plasmid

#147432

Purpose

Insect Expression of DmNot1_1149-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1149-2505-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

6 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNOT1_1-1083_R

Plasmid

#147441

Purpose

Bacterial Expression of DmNot1_1-1083

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1083 (Not1 Fly)

Use

Tags

Expression

Bacterial

Mutation

4 silent mutations and two non silent N605S, K759…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-226_R

Plasmid

#147416

Purpose

Insect Expression of DmNot1_1-226

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-226 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-412_R

Plasmid

#147417

Purpose

Insect Expression of DmNot1_1-412

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-412 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_416-1148_R

Plasmid

#147418

Purpose

Insect Expression of DmNot1_416-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_416-1148 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

3 silent and two non silent N605S, K759M mutation…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2_1-436_R

Plasmid

#147419

Purpose

Insect Expression of DmNot2_1-436

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-436 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R

Plasmid

#147427

Purpose

Insect Expression of DmCCR4_168-552-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552-D412AN414A (twin Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-D412AN414A_R

Plasmid

#147428

Purpose

Insect Expression of DmCCR4-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-D412AN414A (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552_I

Plasmid

#146612

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1-1148_I

Plasmid

#146613

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

four silent and two non silent N605S, K759M mutat…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1717_I

Plasmid

#146614

Purpose

Insect Expression of DmNot1_1147-1717

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1147-2505_I

Plasmid

#146615

Purpose

Insect Expression of DmNot1_1147-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-2505 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and one non silent mutation G1999S compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2_1-401_I

Plasmid

#146616

Purpose

Insect Expression of DmNot2_1-401

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-401 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCR4_168-552_I

Plasmid

#146603

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge